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Hybanthus enneaspermus Muell, belonging to family Violaceae, is a herb or under shrub distributed in the tropical and subtropical regions of the world. It is a herb, often with woody troches, found in the warmer parts of India. the plant is used in case of pregnant and parturient women, and in case of gonorrhoea and urinary infections. The present study is intended to determine the antibacterial activity of the plant against selected urinary tract pathogens and compare it with eight standard drugs frequently used in the treatment of urinary tract infections.